What kind of mucin does PAS stain?
Acid (simple, or non-sulfated) – Are the typical mucins of epithelial cells containing sialic acid. They stain with PAS, Alcin blue at pH 2.5, colloidal iron, and metachromatic dyes.
What type of stain would you use to detect acid mucins and neutral mucins?
The combination of the Alcian blue and the PAS techniques can be used as a means of distinguishing neutral mucins from acid mucins. In most protocols, sections are stained with the standard Alcian blue (pH 2.5) method followed by the PAS technique.
Where are acid mucins found?
The neutral mucins can be found primarily in the surface epithelia of the stomach, Brunner’s glands of the duodenum and in the prostatic epithelium. The acid mucins are found widely distributed throughout the gastrointestinal tract and the respiratory tract.
What is Mucicarmine stain used primarily for?
The mucicarmine staining procedure is very specific in its detection of mucins of epithelial origin. It is often used to identify adenocarcinomas, distinguishing these from squamous-cell carcinomas. It is particularly useful in detecting adenocarcinomas originating from the gastrointestinal tract.
What is PAS stain used for?
The PAS stain is used for demonstration of basement membranes, fungus secreting adenocarcinoma from undifferentiated squamous cell carcinoma, and mucosubstances secreted from the epithelia of various organs. A routine stain for liver and kidney biopsies.
What are the types of mucins?
Histochemically mucins are classified into two types: Epithelial mucin (mucins/mucosubstances) and Connective tissue mucin (mucopolysaccharides). Epithelial mucins are further classified as neutral and acidic.
What does PAS stain for?
The periodic acid-Schiff (PAS) staining procedure is most commonly used in the histology laboratory to detect glycogen deposits in the liver when glycogen storage disease is suspected. Glycogen granules may also be visible in tumors of the bladder, kidney, ovary, pancreas, and lung.
What is alcian blue PAS stain used for?
Alcian Blue/PAS Stain Kit is intended to identify acidic and neutral mucins in tissue sections on the Artisan Link and Artisan Link Pro Staining Systems. This procedure separates the acidic and neutral mucins by color and can be used to distinguish mucins in tissue sections.
What is the function of mucins?
Mucins’ key characteristic is their ability to form gels; therefore they are a key component in most gel-like secretions, serving functions from lubrication to cell signalling to forming chemical barriers. They often take an inhibitory role.
What are acid mucins?
Acid mucins which carry a negative charge on the mucin molecules and can be classified as either simple (carboxyl group added) or complex (sulfuric acid group added). They are found widely throughout the gastrointestinal and respiratory tracts. Neutral mucins which lack acid groups and carry no charge.
Does fungus stain PAS?
The PAS stain is a simple stain that demonstrates polysaccharides. Cellulose and chitin, two substances rich in polysaccharides, are found in the cell walls of fungi. The addition of the enzyme diastase (PASd) eliminates the potential of confounding results caused by the presence of glycogen.
What stains positive for PAS?
Periodic acid–Schiff (PAS) stain highlights the abundant glycogen in the tumor cells; the staining will disappear after diastase treatment. By immunohistochemistry, dysgerminoma cells are positive for PLAP (strong, cytoplasmic staining), SALL4, CD117, and OCT4 (Fig. 16.7).