What is DNA walk?
Genome walking is a method for determining the DNA sequence of unknown genomic regions flanking a region of known DNA sequence. The Genome walking has the potential to capture 6-7 kb of sequence in a single round. Ideal for identifying gene promoter regions where only the coding region.
What is cryptic DNA?
Cryptic genes are phenotypically silent DNA sequences, not normally expressed during the life cycle of an individual. They may, however, be activated in a few individuals of a large population by mutation, recombination, insertion elements, or other genetic mechanisms.
What is Chaos Game Representation?
Chaos Game Representation is a graphical representation of a sequence. It is a method of converting a long one-dimensional sequence (in our case a genetic sequence) into a graphical form.
What is chromosome walking used for?
Chromosome walking is a technique used to clone a gene (e.g., disease gene) from its known closest markers (e.g., known gene) and hence is used in moderate modifications in cloning and sequencing projects in plants, fungi, and animals.
What do you mean by primer walking?
Primer walking is a method to determine the sequence of DNA up to the 1.3–7.0 kb range whereas chromosome walking is used to produce the clones of already known sequences of the gene.
What are cryptic mutations?
Cryptic mutations are undetected base changes in genetic DNA (or hereditary RNA). Some kinds of base change are normally undetected; others may or may not be detected, depending on experimental conditions, procedures and genotypes.
What is the C value paradox and what is its cause?
The so-called C-Value Paradox refers to the observation that genome size does not uniformly increase with respect to perceived complexity of organisms, for example vertebrate with respect to invertebrate animals, or “lower” versus “higher” vertebrate animals (red box).
What is the difference between chromosome walking and jumping?
Chromosomal walking can only sequence and map small lengths of chromosomes while chromosomal jumping enables sequencing of large parts of chromosomes. This is the key difference between Chromosomal walking and chromosomal jumping.
What is meant by chromosome jumping?
Chromosome jumping is a special type of chromosome walking that takes advantage of the breakpoints of chromosomal rearrangements as guide posts and permits the cloning of the two ends of a DNA sequence without the middle section.
What is a Readthrough mutation?
readthrough The continuation of transcription of DNA beyond a normal stop signal, or terminator sequence, due to failure of RNA polymerase to recognize the signal. Readthrough can also occur in translation, when a mutation has converted a normal stop codon into one encoding an amino acid.
What is hidden variation?
5. ΔG is the coefficient of hidden variation. It measures the amount of newly released genetic variation relative to the genetic variation that is expressed on the phenotype under the old conditions, i.e., the relative change in evolvability.
What is the selfish DNA theory?
Selfish-gene theory. The selfish-gene theory of natural selection can be restated as follows: Genes do not present themselves naked to the scrutiny of natural selection, instead they present their phenotypic effects. […] Differences in genes give rise to differences in these phenotypic effects.
Who gave chaos Theory?
Edward Lorenz, from the Massachusetts Institute of Technology (MIT) is the official discoverer of chaos theory.
How is chromosome walking done?
Chromosome Walking and Subtractive Hybridization In this very slow technique, the chromosome of interest is first targeted by FISH (Ch. 5) and isolated by FACs (Ch. 5). The isolated chromosome is then cut by a restriction enzyme and the resulting fragments are used as probes, which will overlap.
What is Oligopaint?
Oligopaint probes are fluorescently-labeled, single-stranded DNA oligonucleotides that can be used to visualize genomic regions ranging in size from tens of kilobases to many megabases.