What is antisense technology in plants?
Antisense technology, which includes antisense RNA. (asRNA) and RNA interference (RNAi), is a gene regulatory strategy that is widely. used for crop improvement. asRNA and RNAi mediated gene silencing is effective. methods for regulating undesirable organic phenomena with no genomic alteration.
What can antisense RNA be used for?
Antisense RNAs play the crucial role in regulating gene expression at multiple levels, such as at replication, transcription, and translation. In addition, artificial antisense RNAs can effectively regulate the expression of related genes in host cells.
What is antisense technology in biotechnology?
Antisense technology is a new and promising tool for controlling gene expression in a cell. Using synthetic antisense oligonucleotides, it targets genes at the level of mRNA, rather than DNA, and prevents them from producing proteins.
How does antisense RNA regulate gene expression?
Antisense RNAs are utilized for gene regulation and specifically target mRNA molecules that are used for protein synthesis. The antisense RNA can physically pair and bind to the complementary mRNA, thus inhibiting the ability of the mRNA to be processed in the translation machinery.
What is the importance of antisense technology in crops or agriculture?
Antisense technology plays an important role in generating high yielding, disease and pest resistant, high nutritional value, and stress-tolerant crop varieties.
How does antisense technology work?
The principle behind antisense technology is that an antisense nucleic acid sequence base pairs with its complementary sense RNA strand and prevents it from being translated into a protein. The oligonucleotide must be nontoxic and sufficiently specific so as not to interfere with other cellular functions.
Why is the antisense strand important?
​Antisense Antisense is the non-coding DNA strand of a gene. In a cell, antisense DNA serves as the template for producing messenger RNA (mRNA), which directs the synthesis of a protein.
Who discovered antisense technology?
Introduction. The potential of oligodeoxynucleotides to act as antisense agents that inhibit viral replication in cell culture was discovered by Zamecnik and Stephenson in 1978 [[1]]. Since then antisense technology has been developed as a powerful tool for target validation and therapeutic purposes.
How can antisense RNA technology be useful in engineering fruit ripening in tomato?
By utilizing ‘antisense RNA’ to modify expression of ripening genes, we have inhibited the production of the cell wall-metabolising enzymes polygalacturonase and pectinesterase and created transgenic plants that contain, effectively, single, targeted mutations affecting these genes.
What is the difference between sense and antisense RNA?
Main Difference – Sense vs Antisense Strand Sense strand contains the exact nucleotide sequence to the mRNA which encodes for a functional protein. Antisense strand serves as the template for the transcription, and contains complementary nucleotide sequence to the transcribed mRNA.
How does antisense RNA inhibit translation?
Most antisense RNAs in bacteria inhibit translation by competing with ribosomes for translation initiation regions (TIRs) on nascent mRNA.