How do you make a solution of polyethylene glycol?
All Answers (4) For a 40% w/v solution, dissolve 40g of PEG 8000 in water or buffer and adjust the volume to 100 mL. For a 40% w/w solution, mix 40g PEG 8000 with 60g water or buffer until you have a solution.
How do you dilute PEG?
Smaller amounts can be made by adjusting the amounts accordingly.
- Measure out 25 grams of PEG 3500 into a 100 mL glass bottle.
- Add 25 mL water and vortex well. Let rest for 10 min.
- Add water to 50 mL total volume.
- Sterilize by autoclave on liquid 20 minute cycle or sterile filter into a new bottle.
How do you make a 50% PEG solution?
50% PEG
- Measure out 25 grams of PEG 3500 into a 100 mL glass bottle.
- Add 25 mL water and vortex well. Let rest for 10 min.
- Add water to 50 mL total volume. Vortex again and let rest until solution looks clear.
- Sterilize by autoclave on liquid 20 minute cycle or sterile filter into a new bottle.
What is polyethylene glycol used for?
Polyethylene glycol 3350 is used to treat occasional constipation. Polyethylene glycol 3350 is in a class of medications called osmotic laxatives. It works by causing water to be retained with the stool. This increases the number of bowel movements and softens the stool so it is easier to pass.
Can I autoclave PEG 8000?
Autoclaving PEG is not recommended (See Sigma Specs), I am assuming that having your PEG at 8000mw is important for your experiment, and if you autoclave it, it will not be anymore. we routinely make up 24% PEG 8000 for virus precipitation and it filter sterilizes just fine at this concentration.
What is PEG in pharmacy?
Polyethylene glycol (PEG) is a synthetic, highly water soluble, inert polymer that is produced in a wide range of molecular sizes and structures. PEGs of various molecular weights have been widely used in cosmetics, pharmaceuticals and other consumer care products.
How much water do you mix with polyethylene glycol?
Mix the PEG 3350 powder in 1/2 to 1 cup (120 to 240 mL) of liquid such as water, juice, soda, or formula. Stir well until the powder dissolves. To promote effectiveness, administer the entire dose, as tolerated, within a maximum of 20 minutes.
Can polyethylene glycol be mixed with water?
If you are using polyethylene glycol 3350 packets, each packet contains a single dose. Pour the powder into a cup containing 8 ounces (240 milliliters) of water, juice, soda, coffee, or tea. Stir to dissolve the powder. Drink immediately.
Can PEG 8000 be autoclaved?