What is the role of the C-terminal domain CTD of the RNA polymerase in RNA processing?
The C-terminal repeat domain (CTD), an unusual extension appended to the C terminus of the largest subunit of RNA polymerase II, serves as a flexible binding scaffold for numerous nuclear factors; which factors bind is determined by the phosphorylation patterns on the CTD repeats.
What is the role of the C-terminal domain CTD of RNA polymerase II in splicing?
CTD facilitates binding of certain splicing factors to splice sites. One hypothesis for the function of the CTD is that it recruits of splicing factors to the site of spliceosome assembly.
What is the CTD of RNA polymerase?
C-terminal domain (CTD) The carboxy-terminal domain (CTD) of RNA polymerase II is that portion of the polymerase that is involved in the initiation of DNA transcription, the capping of the RNA transcript, and attachment to the spliceosome for RNA splicing.
Which of the following is not dependent on the C-terminal domain of RNA polymerase II?
Rna, Transcription, And Translation : Example Question #7 Which of the following is not dependent on the C-terminal domain (CTD) of RNA polymerase II? Explanation: The correct answer is none of the other answers. Only mRNA transcribed by polymerase II undergo 5′ capping, polyadenylation, and splicing.
What is the role of the promoter region in the regulation of gene expression?
The purpose of the promoter is to bind transcription factors that control the initiation of transcription. The promoter region can be short or quite long; the longer the promoter is, the more available space for proteins to bind.
What is the C-terminal domain?
The C-terminal domain of RNA polymerase II functions as a phosphorylation-dependent splicing activator in a heterologous protein. Mol Cell Biol.
Which of the following is not dependent on the C-terminal domain CTD of RNA polymerase II?
How does RNA polymerase find the promoter?
RNA polymerases (or associated general transcription factors) are hypothesized to reach promoter sequences by facilitated diffusion (FD). In FD, a protein first binds to nontarget DNA and then reaches the target by a 1D sliding search.
Why are regions called promoters essential to RNA transcription?
Promoters are a vital component of expression vectors because they control the binding of RNA polymerase to DNA. RNA polymerase transcribes DNA to mRNA which is ultimately translated into a functional protein. Thus the promoter region controls when and where in the organism your gene of interest is expressed.
What is the C-terminal of a protein?
The C-terminus (also known as the carboxyl-terminus, carboxy-terminus, C-terminal tail, C-terminal end, or COOH-terminus) is the end of an amino acid chain (protein or polypeptide), terminated by a free carboxyl group (-COOH).
What happens if CTD is mutated?
Most mutations of the CTD in yeast have pleiotropic effects on one or another major feature of cellular metabolism, including growth rate, cell size, budding pattern, capacity to adjust to physical or metabolic stress, and how efficiently the CTD is phosphorylated by CTD-directed kinases [1], [3], [8], [9].
What controls the binding of the RNA polymerase to the promoter?
general transcription factors
RNA polymerase binds to a promoter with help from a set of proteins called general transcription factors.
Is a subunit of RNA polymerase which recognizes the promoter region?
RNA polymerase synthesizes nucleotide additions only in a 5′ to 3′ direction. The core enzyme has five protein subunits: a dimer of two α proteins, a β protein, a related β′ subunit, and an ω subunit. The β and β′ subunits form the catalytic site, and the α subunit helps recognize the promoter.
What are the N-terminal and C-terminal domains?
Within a peptide, the amine group is bonded to the carboxylic group of another amino acid, making it a chain. That leaves a free carboxylic group at one end of the peptide, called the C-terminus, and a free amine group on the other end called the N-terminus.
What is the C-terminal domain of RNA polymerase II?
The C-Terminal Domain of RNA Polymerase II Functions as a Phosphorylation-Dependent Splicing Activator in a Heterologous Protein NCBI Skip to main content Skip to navigation Resources
Which eukaryotic proteins are required for transcription by RNA polymerase II?
An unusual eukaryotic protein phosphatase required for transcription by RNA polymerase II and CTD dephosphorylation in S. cerevisiae. Mol Cell4: 55–62 [PubMed] [Google Scholar] Komarnitsky P, Cho EJ, Buratowski S 2000. Different phosphorylated forms of RNA polymerase II and associated mRNA processing factors during transcription.
What is the function of RNA polymerase II (RPA II)?
RNA polymerase II is an essential mRNA polyadenylation factor. Nature395: 93–96 [PubMed] [Google Scholar] Hirose Y, Manley JL 2000. RNA polymerase II and the integration of nuclear events. Genes Dev14: 1415–1429 [PubMed] [Google Scholar] Hirose Y, Ohkuma Y 2007.
Which exonuclease promotes transcription termination by RNA polymerase II?
Kim M, Krogen NJ, Vasiljeva L, Rando OJ, Nedea E, Greenblatt J, Buratowski S 2004b. The yeast Rat1 exonuclease promotes transcription termination by RNA polymerase II.