What is single strand conformational polymorphism analysis?
Single-strand conformational polymorphism (SSCP) analysis is a simple and sensitive technique for mutation detection and genotyping. The principle of SSCP analysis is based on the fact that single-stranded DNA has a defined conformation.
What is SSCP in molecular biology?
Single-stranded conformation polymorphism (SSCP) analysis is a widely used screening method that allows you to identify different genomic variants in a large number of samples and in a broad range of organisms, from microorganisms to humans.
What does SSCP mean in medical terms?
Single-strand conformation polymorphism (SSCP), or single-strand chain polymorphism, is defined as a conformational difference of single-stranded nucleotide sequences of identical length as induced by differences in the sequences under certain experimental conditions.
How is the base sequence of DNA determined?
DNA sequencing refers to the general laboratory technique for determining the exact sequence of nucleotides, or bases, in a DNA molecule. The sequence of the bases (often referred to by the first letters of their chemical names: A, T, C, and G) encodes the biological information that cells use to develop and operate.
How does Heteroduplex analysis work?
Hetero-duplex analysis is a low-medium high-throughput SNP detection technique. During heteroduplex analysis, the target gene is PCR amplified and the amplified products are then denatured, re-annealed slowly to form four different double-stranded DNA molecules from the two alleles of the gene (Paniego et al., 2015).
What is heteroduplex PCR?
Heteroduplexes are PCR products that form as a result of cross-hybridization (i.e., recombination) between the competitor sequence and the experimental cDNA.
What is the principle of DNA sequencing?
This method is based on the principle that single-stranded DNA molecules that differ in length by just a single nucleotide can be separated from one another using polyacrylamide gel electrophoresis, described earlier.
Which protein is responsible for heteroduplex formation?
Enzymatic studies indicate that formation of the heteroduplex joint in Escherichia coli involves polymerization of RecA protein on single-stranded DNA (ssDNA) and pairing of the RecA–ssDNA nucleoprotein complex with a double-stranded DNA homologue (reviewed in refs. 1 and 2).
What is a heteroduplex DNA?
A heteroduplex is a double-stranded (duplex) molecule of nucleic acid originated through the genetic recombination of single complementary strands derived from different sources, such as from different homologous chromosomes or even from different organisms.
What is single strand conformational polymorphism?
He further mentioned that Single strand conformational polymorphism is a tool for mutation Glass plates and spacer combs are washed with detection at DNA level. It is a conformation based detergent, ringed under running tap and finally in scanning method of polymorphism detection which is double distilled water before drying.
What is the method of polymorphism detection in detergents?
It is a conformation based detergent, ringed under running tap and finally in scanning method of polymorphism detection which is double distilled water before drying. Before use glass simple and widely used method.
What is the principle of single-stranded DNA (SSCP)?
The principle of SSCP is that the mobility of a single-stranded DNA fragment is dependent on the secondary structure of the fragment. The secondary structure is determined by the nucleotide sequence and the physiological environment (e.g., temperature, pH and ionic strength).
What are the limitations of single point sequence confirmation (SSCP)?
However, the major limitation of SSCP is that, in addition to DNA sequences, many other factors also contribute to the secondary confirmation of DNA complicating the analysis of the obtained results ( Svozil, Kalina, Omelka, & Schneider, 2008 ).