What is MDA cell line?
The MDA-MB-231 cell line is an epithelial, human breast cancer cell line that was established from a pleural effusion of a 51-year-old caucasian female with a metastatic mammary adenocarcinoma1 and is one of the most commonly used breast cancer cell lines in medical research laboratories.
What is the difference between MDA-MB-231 and MDA-MB-468?
MDA-MB-468 is a basal BC cell line while MDA-MB-231 is a claudin-low cell line – a point Hamed Helal made above, to his credit – with claudin-3, claudinin-4 and claudinin-7 low, and typically with low Ki67 and E-cadherin, in contrast to MDA-MB-468 absent these and itself with high Ki-67 and as I noted, MDA-MB-231 shows …
What is the difference between MCF-7 and MDA-MB-231 cell lines?
The MCF-7 cell line has functional estrogen and EGF receptors, is dependent on estrogen and EGF for growth, and is uninvasive, while MDA-MB-231 cells are a model for more aggressive, hormone-independent breast cancer.
What is MDA-MB-468 cell line?
MDA-MB-468 is a cell line with epithelial morphology that was isolated from a pleural effusion of a 51-year-old Black female patient with metastatic adenocarcinoma of the breast. These cells have applications in breast cancer and immuno-oncology research.
Are MDA-MB-231 cells epithelial cells?
MDA-MB-231 are epithelial cells isolated from the breast tissue of a 51-year-old, White, female adenocarcionma patient. Use these cells in your cancer and immuno-oncology research.
How long does it take MDA-MB-231 cells to adhere?
5-7 days
Just aspirate the media and add more. After this, you should see some cells happily attached. Do not change the media or split cells before they are about 60% confluent (which can take 5-7 days for MDA-MB-231, depending on how many survive thawing), unless there are a lot of dead cells floating.
What does MDA-MB-231 mean?
MDA-MB-231 are epithelial cells isolated from the breast tissue of a 51-year-old, White, female adenocarcionma patient. Use these cells in your cancer and immuno-oncology research. Homo sapiens, human. Cell type epithelial cell Morphology epithelial Tissue Breast; Mammary gland Disease. Adenocarcinoma.
Do MCF7 cells express HER2?
Nevertheless, MCF-7 are considered to be moderate EGFR-expressing cell lines (2). Discoveries in breast cancer pathology through experiments using MCF-7 cells. MCF-7 sub-lines demonstrate a wide divergence in the relative expression of ER, PR and HER2.
What is the doubling time for MDA MB 231?
The doubling times for exponentially growing MDA-MB-231 cells were 28.9 h, whereas the doubling times of the eight microcell hybrids varied between 33.4 h (CT60/4) and 44.6 h (CT60/8) (medium 37.7 h) (Figure 2a).
What is A549 cell line?
A549 cells are lung carcinoma epithelial cells that constitute a cell line. A549 is one of the commonly used cell lines for a wide range of research applications.
What is Mammosphere formation?
Mammosphere formation is considered an indicator of a stem-like phenotype in tumor cells and mammosphere assays are regularly used to isolate and culture cancer-initiating cells from a heterogeneous mixture of tumor cells.
What is MDA MB 468 cell line?
Abbreviation / Long Form : MDA-MB 231 / M.D. Anderson – Metastatic Breast 231.
What is the doubling time for MDA-MB-231?
about 38h
Hi Nairrita, the doubling time of MDA-MB-231 cells is about 38h according to ATCC. I usually start with the density of 7,500 cells/cm2 in 96-well plate for 3 days MTS assay.
What is the meaning of Mammosphere?
mammosphere (plural mammospheres) (cytology) A clump of mammary gland cells that forms under certain circumstances.
What are Mammospheres?
Mammospheres, or mammary epithelial stem cell aggregates, derived from primary breast tumors or cell lines are thought to develop from rare cancer stem cell (CSC) subpopulations within the tumor.