What does the size of inoculum mean?
As the previous researchers have stated, inoculum size refers to the initial ratio of microorganisms in relation to the total working volume of the process. For instance, if we look at your stated example, a 5 % v/v inoculum size of a 100ml process would mean you add 5ml of the inoculum to 95ml of the prepared media.
How do you calculate inoculum size?
If you intent is to establish by inoculation 50 liters at 10^5/ml, think you’ll need 500 ml of 10^7/ml inoculum in those 50 liters (i.e. 500ml inoculum in 49.5 liters). Consider: concentration x volume = concentration x volume.
Why is inoculum size important?
Higher inoculum size leads to faster nutrient consumption and thus earlier nutrient depletion compared to lower inoculum size.
How does inoculum size affect microbial growth rate?
The effect of inoculum size and nutrients on bacterial growth was ex- amined using a Biophotometer (Jouan, Paris). The inoculum size greatly affected the length of lag time but not the growth rate. There were two types of reduction of lag time.
What is standard inoculum?
An inoculum can be defined as the population of microorganisms or cells that is introduced in the fermentation medium or any other suitable medium.
What is a heavy inoculum?
Two levels of inoculum were used; the “ heavy ” inoculum was one drop of neat broth culture and the “ light ” inoculum a drop containing approximately 100 organisms. Preparation of desiccates. Suspensions were made by harvesting growth from medium B in 5 per cent.
What is inoculum in microbiology?
An inoculum can be defined as the population of microorganisms or cells that is introduced in the fermentation medium or any other suitable medium. From: Comprehensive Biotechnology (Second Edition), 2011.
How do you calculate CFU ml?
- To find out the number of CFU/ ml in the original sample, the number of colony forming units on the countable plate is multiplied by 1/FDF. This takes into account all of the dilution of the original sample.
- 200 CFU x 1/1/4000 = 200 CFU x 4000 = 800000 CFU/ml = 8 x 10.
- CFU/ml in the original sample.
What is the use of inoculum?
In biology, inoculum refers to the source material used for inoculation. Inoculum may refer to: In medicine, material that is the source of the inoculation in a vaccine. In microbiology, propagules: cells, tissue, or viruses that are used to inoculate a new culture.
What is inoculum of bacteria?
The inoculum is any part of the pathogen that can initiate infection. Thus, in fungi the inoculum may be spores (Figs. 2-3A–2-3C), sclerotia (i.e., a compact mass of mycelium), or fragments of mycelium. In bacteria, mollicutes, protozoa, viruses, and viroids, the inoculum is always whole individuals of bacteria (Fig.
How does inoculum size affect lag phase?
If the lag times of the individual cells are not affected by the inoculum size, then these physiological state values should be scattered around a constant, independently of inoculum size, but with increasing variance as the inoculum size decreases. When data from stationary phase cells inoculated into 1.2 M NaCl (Fig.
What is inoculum density?
The inoculum density (ID) in a sample was estimated as number of microsclerotia per gram of dry soil, and it was recorded for each sample area studied. Symptom severity. Severity of plant symptoms was assessed on a 0-to-100% rating scale adapted from Cirulli et al.