What does cyanine mean?
Definition of cyanine : any of various dyes used especially to sensitize photographic film to light from the green, yellow, red, and infrared regions of the spectrum.
What is cyanine used for?
Cyanine dyes are used to label proteins, antibodies, peptides, nucleic acid probes, and any kind of other biomolecules to be used in a variety of fluorescence detection techniques (Ünlü et al., 1997; Osterman et al., 2013) [5,6]. This section presents synthetic routes to various cyanine dyes.
What Colour is cyanine?
The word cyanin is from the English word “cyan”, which conventionally means a shade of blue-green (close to “aqua”) and is derived from the Greek κυάνεος/κυανοῦς kyaneos/kyanous which means a somewhat different color: “dark blue”.
What does Cy3 stand for?
Cy3 (Sulfo-Cyanine3) is an orange-fluorescent label for protein and nucleic acid (λex=554, λem=568).
What color is Cy3?
Invitrogen Cy3 dye is a bright, orange-fluorescent dye that can be excited using the 532 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. In addition to immunocytochemistry applications, Cy3 dye is commonly used to label nucleic acids.
What is cyanide dye?
Table of Contents. cyanine dye, any member of a class of highly coloured organic compounds used for increasing the range of wavelengths of light to which photographic emulsions are sensitive.
What is a polymethine chain?
: any of a class of dyes (as cyanines or merocyanines) characterized by a resonance structure containing a conjugated chain of carbon atoms that is at least in part an open chain [as in −CH(=CH−CH)n=] attached to two polar atoms (as two amino nitrogen atoms either one of which is considered to be positively charged)
What is FAM PCR?
Applied Biosystems TaqMan Gene Expression assays are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5′ end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3′ end.
What is Black Hole quencher?
Black Hole Quencher™ dyes are true “dark” quenchers with no fluorescent signal, improving signal to noise ratios. Through a combination of FRET and static quenching, they deliver results without the residual background signal common to other quenchers.
What is eriochrome cyanine R (ECR)?
Eriochrome cyanine R (ECR) is a synthetic anionic dye that forms complexes with cations such as iron. We found that an iron-ECR (Fe-ECR) mixture provided either nuclear or myelin staining depending on the differentiator used.
Cyanine is the non-systematic name of a synthetic dye family belonging to polymethine group. The word cyanin is from the English word cyan, which conventionally means a shade of blue-green (close to aqua) and is derived from the Greek κυάνεος/κυανοῦς kyaneos/kyanous which means a somewhat different color: dark blue.
What is Eriochrome Black T?
It is an azo dye. Eriochrome is a trademark of Huntsman Petrochemical, LLC. In its deprotonated form, Eriochrome Black T is blue. It turns red when it forms a complex with calcium, magnesium, or other metal ions.
Are cyanine discs archival?
The ones used are mostly green or light blue in color, and are chemically unstable. For that reason, unstabilized cyanine discs are unsuitable for archival CD and DVD use. Recent cyanine discs contain stabilizers that slow the deterioration significantly. These discs are often rated with an archival life of 75 years or more.